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J Occup Health year 1997 volume 39 number 2 page 113 - 118
Classification Original
Title Separate Determination by Gas-Chromatography of Dimethylformamide, Dimethylacetamide, Monomethylformamide and Monomethylacetamide in Urine for Biological Monitoring*
Author Toshio Kawai1, Kazunori Mizunuma1, Yoko Okada1, Takanori Odachi1, Shun'ichi Horiguchi1, Hiroshi Iguchi2 and Masayuki Ikeda2
Organization 1Osaka Occupational Health Service Center and
2Department of Public Health, Kyoto University Faculty of Medicine
Keywords Biological monitoring, Dimethylacetamide, Dimethylformamide, Monomethylacetamide, Monomethylformamide, Occupational health, Urinalysis
Correspondence
Abstract Separate Determination by Gas-Chromatography of Dimethylformamide, Dimethylacetamide, Monomethylformamide and Monomethylacetamide in Urine for Biological Monitoring: Toshio Kawai, et al. Osaka Occupational Health Service Center-A gaschromatographic method is developed, which allows simultaneous determination of N,N-dimethylformamide (DMF) and N,N-dimethylacetamide (DMA), as well as their metabolites of N-monomethylformamide (MMF) and N-monomethylacetamide (MMA), respectively. Quantitative heat decomposition of N-hydroxymethyl-N-methylacetamide to N-monomethylacetamide required an injection port temperature of 225degree or above, similar to the case of conversion from N-hydroxymethyl-N-methylformamide to N-monomethylformamide. Analysis of urine samples from workers simultaneously exposed to DMF and DMA showed that the separation of small peaks for unidentified materials (which were detected in some urine samples) from that of DMF and DMA was achieved only on a 60 m-long DB-1071 column (and not on a 30 m-long one). The analysis of urine samples from 27 exposed workers showed that both DMF and DMA, in addition to MMF and MMA, were excreted in urine at measurable concentrations. There was a significant correlation between MMF and DMF, and between MMA and DMA; the ratio was about 0.02 to 0.03 for both DMF/MMF and DMA/MMA.