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J Occup Health year 1998 volume 40 number 4 page 302 - 306
Classification Original
Title Increases in DNA and Protein Syntheses in Human Umbilical Vein Endothelial Cells Treated with Asbestos
Author Yuji KARASAKI1, Hajime URANO2, Akira SHIRAHATA2, Ziro NAMBU1, Taro OHJI3, Masayoshi MIURA4 and Sadao GOTOH4
Organization 1School of Health Sciences, 2Department of Pediatrics and 4Biochemistry, School of Medicine, University of Occupational and Environmental Health and 3St. Lucia Hospital
Keywords Asbestos, Human umbilical vein endothelial cell, DNA and protein syntheses
Correspondence
Abstract Increase in DNA and Protein Syntheses in Human Umbilical Vein Endothelial Cells Treated with Asbestos: Yuji KARASAKI, et al. School of Health Sciences, University of Occupational and Environmental Health-When human umbilical vein endothelial cells (HUVECs) were treated with crocidolite asbestos, HUVECs phagocytized the fibers; no cytotoxic effect was observed when treated at concentrations lower than 10 microg/ml for 24 h, 48 h and 72 h. The time-dependent effect of crocidolite asbestos on DNA synthesis and protein synthesis in HUVECs was studied. The DNA synthesis of the cells exhibited the maximum augmentation (150%) at 24 h, and the protein synthesis increased to the maximal value (140%) at 36 h. The dose-dependent effect of crocidolite asbestos on DNA synthesis and protein synthesis in HUVECs was also studied. The maximum increases in DNA synthesis and protein synthesis were observed at 5 microg/ml and at 10 microg/ml of the fibers, respectively. An apparent increase in DNA synthesis may be mainly due to the repair of DNA damage induced by asbestos under our present experimental conditions, but also in part due to the mitogenic effect of asbestos. Cell injury and DNA damage will remain for a while in the cells, and asbestos fibers phagocytized into cells may induce not only specific but also nonspecific gene transcriptions, which might promote mutations in HUVECs and asbestosis.