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J Occup Health year 1999 volume 41 number 4 page 253 - 258
Classification Original
Title Silicon Carbide Induced Pneumoconiosis:
A Microscopic and Biochemical Experimental Study
Author Marilena PETRAN1, Aristotel COCA_RLA1 and Dorin C. OLINICI
Organization 1Hospital of Occupational Medicine Cluj-Napoca,
2Department of Morphopathology, University of Medicine and Pharmacy
Keywords Silicon carbide, Pneumoconiosis, Diffuse interstitial fibrosis, Hydroxyproline, Experiment
Abstract Silicon Carbide Induced Pneumoconiosis: A Microscopic and Biochemical Experimental Study: Marilena PETRAN, et al. Hospital of Occupational Medicine Cluj-Napoca-The pneumoconiogenic effects of silicon carbide were studied in an experimental model by intratracheal instillation of a dose of 50 mg silicon carbide, standardized and commercialized by Duke Scientific Corporation, in a group of 30 guinea pigs killed at 40, 70 and 100 days, respectively. Lung samples were obtained and they were fixed, included, sectioned, stained with hematoxylineosin, van Gieson and Masson's trichromic and studied under the optical microscope. The microscopic aspects were compared with those of the controls (10 guinea pigs), intratracheally instilled with 1 ml physiological solution (NaCl 0.9%) at the beginning of the experiment and killed with the last group of guinea pigs at 100 days. The optical microscopic study showed important changes in the pulmonary structure: alveolar wall ruptures, infiltration of alveolar septa with round cells and histiocytes, as well as diffuse interstitial fibrosis which was more obvious 100 days after the instillation. Lung tissue hydroxyproline, as a marker of collagen synthesis, showed a significant increase correlated with the occurrence of fibrosis. The results of the experimental research support the pneumoconiogenic effect of silicon carbide.