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J Occup Health year 2003 volume 45 number 3 page 168 - 171
Classification Original
Title Urinary PAH Metabolites Influenced by Genetic Polymorphisms of GSTM1 in Male Hospital Incinerator Workers
Author Kyoung-Ho LEE1, 2, Soo-Hun CHO1, 2, Yun-Chul HONG3, Kwan-Hee LEE3, Ho-Jang KWAN4, Inmi CHOI2 and Daehee KANG1, 2
Organization 1Department of Preventive Medicine, Seoul National University College of Medicine,
2Institute for Environmental Medicine, MRC, Seoul National University, 3Department of Preventive Medicine, Inha University College of Medicine and 4Department of Preventive Medicine, Dankook University College of Medicine, Korea
Keywords Polycyclic aromatic hydrocarbon, Hospital incinerator workers, 1-hydroxypyrene glucuronide, Genetic polymorphism, Glutathione S-transferases
Correspondence D. Kang, Department of Preventive Medicine, Seoul National University College of Medicine, Institute of Environmental Medicine, SNUMRC, 28 Yongon-Dong Chongno-Gu, Seoul, 110-799 Korea
Abstract Urinary PAH Metabolites Influenced by Genetic Polymorphisms of GSTM1 in male Hospital Incinerator Workers: Kyoung Ho LEE, et al. Department of Preventive Medicine, Seoul National University College of Medicine, Institute for Environmental Medicine, SNURC Korea-Hospital waste incinerator workers are exposed to various pyrolysis products including polycyclic aromatic hydrocarbons (PAHs). We evaluated their exposure by assessing urinary 1-hydroxypyrene glucuronide (1-OHPG), as an internal dose of PAH exposure. The potential effect of genetic polymorphisms of GSTM1/T1 involved in PAH metabolisms was also investigated. Pre- and post-shift samples were collected from 28 hospital incinerator workers. Urinary 1-OHPG was assayed by synchronous fluorescence spectroscopy (SFS) after immunoaffinity purification with the monoclonal antibody 8E11. Genotypes of GSTM1/T1 were assessed by PCR-based methods. Information on smoking habits and use of personal protective equipment were collected by means of a self-administered questionnaire. The Mann-Whitney test was used to compare group means of these biomarkers. Urinary 1-OHPG levels were similar in pre- and post-shift urine samples. The arithmetic mean concentrations of urinary 1-OHPG were 0.16 plusmn 0.04 micromol/mol creatinine pre-shift and 0.19 plusmn 0.09 micromol/mol creatinine post-shift, but urinary 1-OHPG levels were significantly higher in individuals with the GSTM1 null genotype than with the GSTM1 present genotype (p=0.05, by Mann-Whitney test). Our results suggest that the urinary 1-OHPG levels in hospital waste incinerator workers may be modified by the GSTM1 genotype, but these findings remain to be confirmed in future studies involving larger sample sizes.