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J Occup Health year 2004 volume 46 number 3 page 175 - 180
Classification Original
Title Occupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography-Mass Spectrometry Method
Author Grazyna BIENIEK, Slawomir KURKIEWICZ and Tadeusz WILCZOK
Organization Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Poland
Keywords GC/MS analysis, Mixed exposure, Aromatic hydrocarbons, Urinary metabolites
Correspondence G. Bieniek, Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Jagiellonska 4, 41-200 Sosnowiec, Poland (e-mail: maja@slam.katowice.pl)
Abstract Occupational Exposure to Aromatic Hydrocarbons at a Coke Plant: Part I. Identification of Hydrocarbons in Air and their Metabolites in Urine by a Gas Chromatography-Mass Spectrometry Method: Grazyna BIENIEK, et al. Department of Molecular Biology, Biochemistry and Biopharmacy, Faculty of Pharmacy, Medical University of Silesia, Poland-A method for the qualitative analysis of aromatic hydrocarbons in air and their various urinary metabolites is presented. The air was sampled in charcoal tubes and extracted with carbon disulfide. The hydrocarbons were identified as being aliphatic hydrocarbons (C9-C19), aromatic hydrocarbons and heterocyclic compounds. The urinary metabolites after enzymatic hydrolysis were analyzed by solid-phase extraction with a styrene-divinylbenzene resin, silylation with N,O-bis(trimethylsilyl)acetamide and GC/MS for separation and detection. Satisfactory separation of all compounds investigated was achieved without interference due to matrix peaks. The following compounds were identified in the urine of workers: dimethylphenol isomers, 4-ethyl-1,3-benzenediol, 2-ethoxybenzoic acid and methoxyphenols. Trimethylsilyl derivatives of aromatic hydroxyacids and hydroxymethoxyacids were found in the urine of occupationally exposed workers by means of a silylation procedure.