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J Occup Health year 2004 volume 46 number 5 page 359 - 364
Classification Original
Title Effects of Prednisolone and Complex of Vitamin B1, B2, B6 and B12 on Organophosphorus Compound-Induced Delayed Neurotoxicity
Author Fengyuan PIAO1, Ning MA2, Hidetaka YAMAMOTO3 Toru YAMAUCHI1 and Kazuhito YOKOYAMA1
Organization 1Department of Public Health, School of Medicine, Mie University, 2The Second Department of Anatomy, School of Medicine, Mie University and 3Department of Forensic Medicine and Sciences, School of Medicine, Mie University, Japan
Keywords Organophosphorus compound-induced delayed neurotoxicity (OPIDN), Tri-o-cresyl phosphate (TOCP), Leptophos, Vitamin B complex, Synthetic adrenal cortical hormone, Delayed neuropathy
Correspondence F. Piao, Department of Public Health, School of Medicine, Mie University, 2-174 Edobashi, Tsu-shi, Mie 514-8507, Japan (e-mail:
Abstract Effects of Complex of Prednisolone and Vitamin B1, B2, B6 and B12 on Organophosphorus Compound-Induced Delayed Neurotoxicity: Fengyuan PIAO, et al. Department of Public Health, School of Medicine, Mie University-Protective effects of prednisolone as a synthetic adrenal cortical hormone and complex of vitamin B1, B2, B6 and B12 on organophosphorus compound-induced delayed neurotoxicity (OPIDN) caused by leptophos and tri-o-cresyl phosphate (TOCP) as organophosphates (OPs) were examined. Nine groups of hens (six for each) were used. Eight groups received intravenous injection of 30 mg/kg of leptophos or 40 mg/kg of TOCP (four groups in each). Among them, three groups which received leptophos were given (p.o.) predonisolone (2 mg/body), vitamin B complex (25 mg/body) or both 3 h after OPs injection and then every day for 15 d (one group for each); the same treatment was performed on three groups which received TOCP. The remaining one group served as controls. It was observed that delayed neuropathy induced by OPs could not be resisted completely by the treatment with prednisolone or vitamin B complex, but clinical signs of OPIDN and pathological changes in hens that received these two protective agents after OPs were less severe than those in hens that received only OPs. Of these groups, the improvement in clinical signs was best shown in hens that received the both two protective agents. In addition, improvement in clinical signs among the hens that did not deteriorate to paralysis was observed. In particular, those which developed mild ataxia recovered well. It is indicated that combining administration of prednisolone and vitamin B complex early before clinical signs are manifest is effective in alleviating neuropathy. It is also suggested that recovery or good prognosis will be expected, as long as progression of the clinical signs is prevented before paralysis develops in delayed neuropathy.