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J Occup Health year 2005 volume 47 number 1 page 56 - 60
Classification Original
Title Ceruloplasmin Oxidase Activity as a Biomarker of Lead Exposure
Author Warin LEELAKUNAKORN1, Rosawan SRIWORAWIT2 and Suganya SOONTAROS2
Organization 1Department of Pathology, Faculty of Medicine, Mahidol University and 2Department of Biochemistry, Faculty of Science, Chulalongkorn University, Thailand
Keywords Lead, Ceruloplasmin, Oxidase activity
Correspondence S. Soontaros, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Payathai Road, Bangkok 10330, Thailand
(e-mail: suganya.s@chula.ac.th)
Abstract Ceruloplasmin Oxidase Activity as a Biomarker of Lead Exposure: Warin LEELAKUNAKORN, et al. Department of Pathology, Faculty of Medicine, Mahidol University, Thailand-The most sensitive method used in monitoring the blood lead-level is atomic absorption spectrophotometry. Its limitation due to its high cost has made delta-aminolaevulinic acid dehydratase (delta-ALAD) inhibition more popular. This work demonstrates that ceruloplasmin (Cp), the blue-colored copper containing alpha-2 globulin (alpha2 globulin) glycoprotein in serum, can serve as a high-potential biomarker for lead exposure. Sephadex G-200 column chromatography demonstrated that lead-intoxicated patient serum transferrin and ceruloplasmin are bound to lead. The ceruloplasmin's oxidase and delta-ALAD activities are reduced when the blood-lead level increases (thresholds of 10.0 and 12.5 microg/100 mL respectively), with a correlation coefficient of -0.8643 and -0.6785 which accurately estimate a blood lead level of 74.7% and 46.0%, respectively. When the blood-lead level was less than 20 microg/100 mL, correlation coefficients of -0.5785 and -0.6963 suggested that Cp may serve as a good biomarker. Other advantages in using Cp in monitoring the blood-lead level were its stability: the enzyme was more stable at -20C, for more than 2 wk, and its practical and rapid determination procedure.