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J Occup Health year 2007 volume 49 number 5 page 389 - 398
Classification Original
Title Oxidative DNA Damage Estimated by Plasma  8-hydroxydeoxyguanosine (8-OHdG): Influence of  4, 4'-methylenebis (2-chloroaniline) Exposure and Smoking
Author Hong-I CHEN1, Saou-Hsing LIOU2, 3, Shu-Fen HO2, Kuen-Yuh WU3, Chien-Wen SUN3,
Ming-Fong CHEN3, Li-Chuan CHENG3, Tung-Sheng SHIH4 and Ching-Hui LOH5
Organization 1Division of Urology, Department of Surgery, Tri-Service General Hospital, 2Department of Public Health, National Defense Medical Center, 3Division of Environmental Health and Occupational Medicine, National Health Research Institutes, 4Institute of Occupational Safety and Health, Council of Labor Affairs and China Medical University and Hospital and 5Department of Family Medicine & Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taiwan, R.O.C
Keywords 4, 4'-methylenebis (2-chloroaniline) (MBOCA), Oxidative DNA damage, 8-hydroxydeoxyguanosine (8-OHdG), Smoking, Liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS)
Correspondence C.-H. Loh, Department of Family Medicine, Tri-Service General Hospital, National Defense Medical Center, 325 Chen-Kung Rd., Sec. 2, Neihu, Taipei, Taiwan 114, Republic of China (e-mail: twdoc@ndmctsgh.edu.tw)
Abstract Oxidative DNA Damage Estimated by Plasma 8-hydroxydeoxyguanosine (8-OHdG): Influence of 4, 4'-methylenebis (2-chloroaniline) Exposure and Smoking: Hong-I CHEN, et al. Division of Urology, Department of Surgery, Tri-Service General Hospital, Taiwan, R.O.C-Oxidative DNA damage may play an important role in the human carcinogenic process. Recently, we reported a case of bladder cancer among 4, 4'-methylenebis (2-chloroaniline) (MBOCA)-exposed workers. By measuring the plasma level of 8-hydroxydeoxyguanosine (8-OHdG), we investigated the association between oxidative DNA damage and MBOCA exposure. In addition, we examined the effects of different confounders on the plasma level of 8-OHdG. We undertook a cross-sectional survey at four MBOCA-producing factories in Taiwan (158 subjects). Plasma 8-OHdG levels and urinary MBOCA concentrations were measured by liquid chromatography coupled with tandem mass spectrometry (LC/MS/MS). Personal characteristics were collected by questionnaire. The workers were classified according to their job titles as exposed (n=57) or unexposed (n=101) groups as well as classified according to urinary MBOCA levels as high urinary MBOCA (>20microg/g creatinine) (n=45) or low urinary MBOCA (n=108) groups. Neither the MBOCA-exposed workers nor the high urinary MBOCA workers had a significant increase in the mean plasma 8-OHdG level, even after adjustment for potential confounders. Age and gender were significantly positively correlated with plasma 8-OHdG levels. Smokers among high urinary MBOCA workers also had significantly higher 8-OHdG levels than non-smokers among high urinary MBOCA workers. Our study provides evidence that smoking rather than MBOCA exposure induces elevation of plasma 8-OHdG levels among workers exposed to MBOCA, indicating that oxidative DNA damage does not play an important role in the carcinogenic processes of MBOCA.